Universität Regensburg

Christoph Engel

Uni Regensburg

Anschrift:

Leistungsprofil:

• Transkription durch RNA polymerase I

• Transkription durch RNA polymerase I

• (Cryo-)Elektronenmikroskopie inkl. computergestützte Datenprozessierung und 3D-Modellierung
• Proteinkristallographie
• Biophysikalische Methoden
• Chromatographiemethoden (FPLC)
• In-vitro Transkriptionsanalyse

Publikationen:

• Complete List: https://scholar.google.de/citations?hl=de&user=dje66YkAAAAJ
• Florian B. Heiss, Julia L. Daiß, Philipp Becker, and Christoph Engel$. Conserved strategies of RNA polymerase I hibernation and activation. Nature communications 12, 758 (2021)
• Ewan P Ramsay*, Guillermo Abascal-Palacios*, Julia L Daiß*, Helen King, Jerome Gouge, Michael Pilsl, Fabienne Beuron, Edward Morris, Philip Gunkel, Christoph Engel$, Alessandro Vannini$ Structure of human RNA polymerase III. Nature communications 11, 6409 (2020)
• Michael Pilsl, and Christoph Engel$ Structural basis of RNA polymerase I pre-initiation complex formation and promoter melting. Nature Communications 11, 1206 (2020)
• Kevin Kramm, Tim Schröder, Jerome Gouge, Andrés M Vera, Kapil Gupta, Florian B Heiss, Tim Liedl, Christoph Engel, Imre Berger, Alessandro Vannini, Philip Tinnefeld, Dina Grohmann DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability. Nature Communications 11, 2828 (2020)
• Philipp Merkl, Michael Pilsl*, Tobias Fremter, Katrin Schwank, Christoph Engel, Gernot Längst, Philipp Milkereit, Joachim Griesenbeck and Herbert Tschochner RNA polymerase I (Pol I) passage through nucleosomes depends on Pol I subunits binding its lobe structure Journal of Biological Chemistry RA119. 011827
• Kevin Kramm, Christoph Engel, and Dina Grohmann Transcription initiation factor TBP: old friend new questions. Biochemical Society Transactions 47 (1), 411-423 (2019)
• Christoph Engel$, Simon Neyer, and Patrick Cramer$ Distinct Mechanisms of Transcription Initiation by RNA Polymerases I and II. Annu Rev Biophys 2018. 47, 425-446
• Christoph Engel*, Tobias Gubbey*, Simon Neyer*, Sarah Sainsbury, Christiane Oberthuer, Carlo Baejen, Carrie Bernecky, and Patrick Cramer Structural basis of RNA polymerase I transcription initiation. Cell, 2017, 169(1), 120-131.
• Christoph Engel, Jürgen Plitzko, and Patrick Cramer RNA polymerase I-Rrn3 complex at 4.8 Å resolution. Nature Communications, 2016 Jul 15;7:12129. Christoph Engel$ Purification of crystallization-grade RNA polymerase I from S. cerevisiae. Methods Mol Biol, 2016, 1455, 85-97. --- Simon Neyer*, Michael Kunz*, Christian Geiss, Merle Hantsche, Victor-Valentin Hodirnau, Anja Seybert, Christoph Engel, Margot Scheffer, Patrick Cramer, and Achilleas S. Frangakis Structure of RNA polymerase I transcribing rDNA gene. Nature, 2016, 540 (7634), 607-610. --- Ana Lisica, Christoph Engel*, Marcus Jahnel*, Eric A. Galburt*, Edgar Roldan, Patrick Cramer and Stephan W. Grill: Mechanisms of backtrack recovery by RNA polymerases I and II. Proc Natl Acad Sci U S A, 2016, Mar 15;113(11):2946-51. --- Dirk Kostrewa*, Claus Kuhn*, Christoph Engel and Patrick Cramer: An alternative RNA polymerase I structure reveals a dimer hinge. Acta Crystallogr D, 2015, Sep;71(Pt 9):1850-5. --- Christoph Engel, Sarah Sainsbury, Alan C. Cheung, Dirk Kostrewa and Patrick Cramer: RNA polymerase I structure and transcription regulation, Nature, 2013, 502(7473):650-5. --- Gert Bange*, Nico Kuemmerer*, Christoph Engel, Gunes Bozkurt, Klemens Wild and Irmgard Sinning: FlhA provides the adaptor for coordinated delivery of late flagella building blocks to the type III secretion system, Proc Natl Acad Sci U S A, 2010 Jun 22; 107(25):11295-300.

Kooperationsangebot für die Wirtschaft / Praxis:

• Therapeutische Beeinflussung der enzymatischen Ativität der RNA Polymerase I
• Struktur-basierte Compound (Weiter-)Entwicklung